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Applications: Positive control in the transduction of neuronal cells.Optimization of transduction assays and tracking of transgene expression over time.
Background: Adeno-Associated Virus-DJ (AAV-DJ) is a synthetic serotype made from eight different wild-type AAV serotypes (AAV2, 4, 5, 8, 9, avian, bovine, and goat AAV) using DNA shuffling. These modifications allow the AAV-DJ serotype to exhibit improved transduction efficiency in vitro and in vivo and infect a broader range of cell types compared to the wild-type serotypes. Synapsin I is a neuron-specific phosphoprotein that coats the cytoplasmic surface of small synaptic vesicles and is involved in axonogenesis and synaptogenesis. Mutations in this protein can result in X-linked neuronal degenerations (example: Rett Syndrome). The proximal region of the synapsin I promoter is sufficient to direct neuron-specific gene expression. The synapsin I promoter has been used to achieve neuron-specific long-term transgene expression in vivo.
Description: These AAV-DJ particles constitutively express the firefly (Photinus pyralis) luciferase under the control of a SYN1 promoter.
Formulation: AAV was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer containing 0.01% Pluronic F68. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Purification: The purity of the AAV particles was confirmed to be greater than 90% by staining with One-Step Lumitein™ UV Protein Gel Stain (Biotium, 21005-1L). The purity varies with each lot; the exact value will be provided with each shipment.
Storage Stability: AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (50 µl x 2) of AAV at a titer ≥1 x 1012 vector genomes/ml. The titer is determined by qPCR and varies with each lot; the exact value will be provided with each shipment.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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