AAV1 ZsGreen

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Applications: Use as a positive control for transductionOptimize transduction assays and track expression over time

Description: Adeno-Associated Virus Serotype 1 (AAV1) exhibits high homology with other AAV serotypes. AAV1 efficiently transduces muscle tissue, as determined by a region of the capsid protein VP1 (amino acids 350 to 430) which functions as a major determinant of tissue tropism.These AAV1 particles constitutively express ZsGreen under a CMV promoter. ZsGreen is a human codon-optimized variant of the green fluorescent protein isolated from reef coral (Zoanthus sp). It has been engineered for higher expression in mammalian cells and is up to four times brighter than enhanced GFP (eGFP). ZsGreen expression and AAV1 transduction efficiency can easily be verified and optimized by fluorescence microscopy or flow cytometry. ZsGreen has an excitation wavelength of 493 nm and an emission wavelength of 505 nm.

Formulation: AAV1 was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer with 0.01% Pluronic F68.

Purification: The purity of the AAV particles was confirmed to be greater than 90% by staining with One-Step Lumitein™ UV Protein Gel Stain (Biotium #21005-1L). The purity will vary with each lot; the exact value is provided with each shipment.

Storage Stability: AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80°C. 

Supplied As: Two vials (50 µl x 2) of AAV at a titer ≥1 x 10¹² TU/ml. The titer is determined by qPCR and will vary with each lot; the exact value is provided with each shipment.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1

• SKU: BPS78443
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 78443
• Package Unit: 50 µl x 2
• Quantity Unit: PAK