AAV6 Luciferase-eGFP

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Applications: Use as a positive control for transductionOptimize transduction assays and track protein expression over time

Description: Adeno-Associated Virus serotype 6 (AAV6) appears to be related to AAV1 by sequence analysis and shows the highest transduction efficiency in pancreatic beta-cells compared to other AAV serotypes.These AAV6 particles constitutively express the firefly (Photinus pyralis) luciferase and enhanced Green Fluorescent Protein (eGFP) genes connected via a T2A linker, under the control of a CMV promoter. The T2A self-cleaving peptide (derived from Thosea asigna virus 2A) leads to the efficient cleavage of the transcript, and expression of luciferase and eGFP as two separate proteins.

Formulation: AAV6 was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer containing 0.01% Pluronic F68.

Purification: The purity of the AAV particles was confirmed to be greater than 90% by staining with One-Step Lumitein™ UV Protein Gel Stain (Biotium #21005-1L). The purity will vary with each lot; the exact value will be provided with each shipment.

Storage Stability: AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80°C. 

Supplied As: Two vials (50 µl x 2) of AAV at a titer ≥1 x 10¹² TU/ml. The titer is determined by qPCR and will vary with each lot; the exact value will be provided with each shipment.

Warnings: Avoid freeze/thaw cycles. 

Biosafety Level: BSL-1

• SKU: BPS78466
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 78466
• Package Unit: 50 µl x 2
• Quantity Unit: PAK