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Applications: Positive control for anti-CD19 CAR evaluation in T cells.Transduction optimization experiments.Generate anti-CD19 CAR-T cells (for research use only, not for therapeutic purposes).
Background: CD19 (also known as Cluster of Differentiation 19, B-lymphocyte surface antigen B4, or CVID3) is a glycoprotein expressed at the surface of B lymphocytes through most phases of B cell maturation. It is strictly required for B cell terminal differentiation. Mutations in the CD19 gene cause severe immune-deficiency syndromes associated with impaired antibody production such as CVID3 (common variable immuno-deficiency 3). The majority of B cell malignancies express normal to high levels of CD19, making it a nearly ideal target for cancer immunotherapy. Blinatumomab, a CD19/CD3 bi-specific T cell engager (BiTE) has been approved for relapsed/refractory B precursor ALL (Acute lymphoblastic leukemia) and CD19 was the target of the first approved CAR-T cell therapy. Studies of CD19 function and expression profiles will continue to broaden our knowledge and support broader applications in cancer therapy.
Description: The anti-CD19 CAR lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most mammalian cells, including primary and non-dividing cells. These viruses transduce cells with the ScFv portion of anti-CD19 (clone FMC63) linked to the 2nd generation CAR (Chimeric Antigen Receptor), containing CD8 hinge and transmembrane domains, 4-1BB and CD3ζ signaling domains. The lentiviruses also include a puromycin selection marker.Note: This product transduces the same anti-CD19 CAR construct (CD19 ScFv-CD8-4-1BB-CD3ζ) as other available BPS Bioscience anti-CD19 CAR lentiviruses (BPS Bioscience #78600,78601 and 78775) but differs in key aspects. This product contains a puromycin selection marker that can be used for selection. BPS Bioscience Catalog # Self-Inactivation (SIN) Selection Marker78600 no puromycin 78601 yes no78602 yes puromycin78775 yes eGFP
Formulation: The lentiviruses were produced from HEK293T cells, concentrated, and resuspended in DMEM. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: 50 µl of anti-CD19 CAR at a titer ≥ 3x108 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Uniprot: P15391
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
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