Products from BPS Bioscience require a minimum order value above 400€Applications: Positive control for anti-mesothelin CAR evaluation in T cells; useful for transduction optimization.
Background: Mesothelin (MSLN) is a glycophosphatidylinositol (GPI) linked cell-surface protein that is produced as a ~70 kDa precursor protein and cleaved by Furin protease to generate the ~40 kDa mature form. MSLN is frequently overexpressed in mesothelioma, ovarian, pancreatic, and non-small cell lung cancers, while its expression in normal tissues is restricted to the mesothelial lining. MSLN is a tumor-associated antigen and has been an attractive target for targeted immunotherapy, including drug-conjugated antibodies and chimeric antigen receptor T cells (CAR-T Cells).
Description: The Anti-mesothelin CAR lentiviruses are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce the ScFv (single-chain fragment variable) of anti-mesothelin (Clone P4) linked to a 2
nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains (Figure 1).A
B
Figure 1. (A) Schematic of the lenti-vector used to generate the anti-mesothelin CAR lentivirus and (B) Construct diagram showing components of the anti-mesothelin CAR.
Formulation: The lentiviruses were produced from HEK293T cells, concentrated, and resuspended in DMEM.
Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C.
Supplied As: 50 µl of anti-mesothelin CAR at a titer ≥ 3x10
8 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Uniprot: Q13421
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
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