Products from BPS Bioscience require a minimum order value above 400€Applications: Screen for activators or inhibitors of the JNK signaling pathway in the transduced target cells.Generate an AP1 eGFP Reporter stable cell line (geneticin-resistant).
Background: The stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) family of proteins are activated by stress, inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and morphogenesis. Upon activation of the SAPK/JNK pathway, MAP Kinase Kinases phosphorylate and activate JNKs. The activated JNKs translocate to the nucleus where they phosphorylate and activate transcription factors such as c-Jun, c-Fos, ATF (Activating transcription factor), and JDP (Jun dimerization protein). These transcription factors form heterodimers termed AP1, which bind to AP1 response elements in the promoters of target genes and induce their transcription.
Description: The Activator protein 1 (AP1) eGFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an eGFP gene driven by an AP1 response element located upstream of the minimal TATA promoter (Figure 1). After transduction, activation of the JNK signaling pathway and AP1-mediated activity in the target cells can be monitored by eGFP expression.
Figure 1. Schematic of the lenti-vector used to generate the AP1-eGFP Reporter Lentivirus.
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer >10
7 TU/ml. The titer will vary with each lot; the exact value will be provided with each shipment.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
English
Deutsch
