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Applications: Expression of ATF6-dependent luciferase reporter to study the ATF6 response Generate ATF6 luciferase reporter stable cell lines (puromycin resistant).
Background: Activating Transcription Factor 6 (ATF6) is a transmembrane transcription factor that responds to endoplasmic reticulum (ER) stress via the unfolded protein response (UPR). In the presence of excess misfolded proteins, the UPR is activated and there is an increase in chaperone expression. ATF6 is one of the three critical proteins of the UPR. In response to ER stress, ATF6 is cleaved, and the cytosolic portion is translocated to the nucleus where it binds to ER stress-response elements on the promoters of target genes, leading to transcription of ER molecular chaperones. Dysfunction in the UPR pathway results in developmental defects, neurodegenerative diseases, and cancer. Strategies targeting ATF6 may prove beneficial for cancer and degenerative disease treatment.
Description: The ATF6 (Activating Transcription Factor 6) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce most types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with the firefly luciferase gene driven by multiple copies of an ATF6 response element, located upstream of the minimal TATA promoter. The lentiviruses also transduce a puromycin selection gene (Figure 1). After transduction, the ATF6-mediated response to ER (endoplasmic reticulum) stress can be monitored by measuring luciferase activity.
Formulation: The lentiviruses were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer >107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
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