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Applications: Study CD8-MHC class I interaction.Generation of a stable cell line expressing CD8a and CD8b with puromycin selection.
Description: CD8 (cluster of differentiation 8) is a cell surface glycoprotein found on most cytotoxic T lymphocytes that functions within the immune system to mediate cell-cell interactions. The functional forms of CD8 consist of either a heterodimer of two isoforms, CD8α and CD8β, or a homodimer of two CD8α molecules. CD8 acts as a coreceptor to facilitate binding between the T-cell Receptor (TCR) and the class I Major Histocompatibility Complex (MHC). Studying CD8 and its importance in isoimmunity can further our understanding of post-transplant recognition.The CD8a/CD8b (CD8α/CD8β) Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain P2A-linked CD8a (NM_001768.6) and CD8b (NM_004931.5) driven by an EF1a promoter and a puromycin selection marker).
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Uniprot: CD8a: P01732; CD8b: P10966
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
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