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Application: Expression of human CDH17 in cells of interest.Generate CHD17-expressing cell pools or stable cell lines following puromycin selection.
Background: CHD17 (cadherin 17), also known as LI (liver-intestine)-cadherin is a unique member of the cadherin superfamily of proteins, as it has seven instead of the five typical cadherin domains. It is a calcium-dependent membrane-associated glycoprotein normally expressed on epithelial cells of the small intestine and colon, where it regulates intercellular adhesion. Upregulation of this protein is found in gastric cancer, colorectal and pancreatic cancer, amongst others. CHD17 has become a therapeutic target of interest, with studies using monoclonal antibodies, ADC (antibody drug conjugates) and CAR-T cells resulting in promising outcomes. More recently nanobodies, with their smaller size and higher potential to penetrate tumors, have also been developed. A bispecific T cell engager, ARB202, has also shown great promise in vitro. CHD17 is thus a target with great potential and future studies will continue to open new avenues of treatment around it.
Description: The CDH17 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with human CDH17 (cadherin 17) (NM_004063.4) driven by a CMV promoter. The lentiviruses also transduce a puromycin selection marker.
Formulation: The lentivirus particles were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
References: Ma J., et al., 2022 Biomater Res 26:24.
Wong D., et al., 2021 Pancreatic Cancer 39 (3): 405.
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