Products from BPS Bioscience require a minimum order value above 400€Applications: Generation of a stable cell line expressing human EpCAM with puromycin selection
Background: Epithelial cell adhesion molecule (EpCAM, also known as CD90) is specific to epithelial cells and regulates cell proliferation and differentiation. It is used as an epithelial cell biomarker to detect circulating tumor cells. EpCAM belongs to the small GA733 protein family and is a homophilic transmembrane glycoprotein involved in cell-cell adhesion and tissue plasticity. It is a single-chain membrane-spanning protein with three major domains known as EpEX, involved in epidermal growth factor receptor (EGFR) mediated signaling pathways. Due to the versatile function of EpCAM and its crosstalk with other signaling pathways in cancer, EpCAM is considered an attractive target for cancer diagnosis, prognosis, and therapy.
Description: The EpCAM Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. The particles contain a human EpCAM (NM_002354.3) driven by an EF1A promoter and a puromycin selection marker (Figure 1).
Figure 1: Schematic of the lenti-vector used to generate the EpCAM Lentivirus
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥10
7 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Uniprot: P16422
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
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