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Application: Negative control in experiments involving lentiviral transduction of TET-On™ expression vectors.Generate negative-control cell pools or stable cell lines following G418 selection, to validate the specificity of the effects observed by inducible expression of a protein of interest.
Background: The use of inducible expression systems is common in cell biology, as it allows for temporal control of gene expression, since expression can be reversibly turned on and off. In the case of the TET-On™, a tetracycline-controlled transcriptional activation, treatment with tetracycline or a derivative can turn the system on. TRE (tetracycline response element) is usually upstream of a minimal promoter, which per se has very low expression, and shows very tightly regulated expression.
Description: Expression Negative Control Lentivirus (Inducible Tet On™) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viral particles do not express any specific protein under a tight TRE tetracycline-inducible promoter but include a G418 selection marker.
Formulation: The lentivirus particles were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
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