Products from BPS Bioscience require a minimum order value above 400€Applications: Generate a stable cell line expressing human FcRL5 with puromycin selection
Background: FcRL5 (Fc receptor-like protein 5) is a single-pass transmembrane protein containing 8 immunoglobulin-like C2-type domains in its extracellular portion, and a short cytoplasmic tail. FcRL5 is expressed in B cells and may be involved in B cell development and lymphomagenesis. Moreover, it is enriched in malignant plasma cells of patients diagnosed with multiple myeloma and is an attractive target for antibody-drug conjugates (ADC) and anti-FcRL5 CAR T cells.
Description: The FcRL5 Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. The particles contain a human FcRL5 (NM_031281.3) driven by an EF1A promoter and a puromycin selection marker (Figure 1).
Figure 1: Schematic of the lenti-vector used to generate the FcRL5 Lentivirus.
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥10
7 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Uniprot: Q96RD9
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
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