Firefly Luciferase Lentivirus (EF1A Promoter/Geneticin, Hygromycin, or Puromycin) -78740-P

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Application: Use as a positive control for transduction.Optimize transduction assays.Generate a stable cell line expressing Firefly Luciferase with Puromycin.

Description: The Firefly Luciferase Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The viruses transduce firefly luciferase under the control of an EF1A promoter.Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase Lentivirus (Geneticin).Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase Lentivirus (Hygromycin).Figure 1. Schematic of the lenti-vector used to generate the Firefly Luciferase Lentivirus (Puromycin).

Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.

Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C. 

Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-2

• SKU: BPS78740-P
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 78740-P
• Package Unit: 500 µl x 2
• Quantity Unit: STK