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Applications: Screen for activators or inhibitors of IFN-γ-induced signal transduction pathways.Generate GAS Luciferase Reporter stable cell line (puromycin resistant).
Background: Interferon gamma (IFN-γ) is a pleiotropic cytokine with anti-viral, anti-tumor, and immune-modulatory functions. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, Interferon gamma receptor 1 (IFNGR1) and Interferon gamma receptor 2 (IFNGR2), associated with kinases JAK1 and JAK2, respectively. Upon binding to this receptor, IFN-γ triggers JAK/STAT signaling. The activated STAT1 homodimers translocate to the nucleus where they bind interferon-gamma-activated sites (GAS) in the promoter of IFN-γ inducible genes, which stimulates IFN-γ-specific activation of gene transcription.
Description: The GAS Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by three copies of the interferon gamma (IFN-γ) activated sites (GAS) located upstream of the minimal TATA promoter and a puromycin selection gene for the selection of stable clones. After transduction, the GAS-regulated gene expression in the target cells can be monitored by measuring the luciferase activity.
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer >107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
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