HSE Luciferase Reporter Lentivirus (Heat Shock Response)

Products from BPS Bioscience require a minimum order value above 400€

Applications: Expression of HSF1-dependent luciferase reporter to study the heat shock response. Generate HSE Luciferase Reporter stable cell lines (puromycin resistant).

Background: The Heat Shock Response (HSR) is crucial for cells to adapt to stressful conditions. In the presence of high temperatures, oxidative stress or heavy metals, several proteins can unfold and become unable to perform their normal functions. This in turn can result in cell damage and/or death. To maintain proteins with the proper structure the expression of Heat Shock Proteins (HSP) is induced. Expression of HSPs is regulated by the transcription factor Heat Shock Factor 1 (HSF1). When cellular stress occurs HSF1 undergoes a confirmation change and moves to the nucleus, where it can bind to Heat Shock Elements (HSE) and lead to transcription of the HSP mRNA. Dysfunction in the HSR can lead to pathologies such as neurodegenerative diseases and cancer. Targeting HSE may prove beneficial in the treatment of pathologies linked to defective HSR.

Description: The HSE (Heat Shock Response) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce most mammalian cells, including primary and non-dividing cells. These viruses transduce cells with the firefly luciferase gene driven by multiple copies of the heat shock response element (HSE) located upstream of the minimal TATA promoter. The lentiviruses also transduce a puromycin selection gene.  After transduction, the heat shock response in the target cells can be monitored by measuring luciferase activity.

Formulation: The lentiviruses were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.  

Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Supplied As: Two vials (500 µl x 2) of lentivirus at a titer >10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-2

• SKU: BPS78669
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 78669
• Package Unit: 500 µl x 2
• Quantity Unit: STK