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Applications: Use as a positive control for IAV HA TCR evaluation and optimize experimental conditions for influenza research.Generate IAV HA TCR expression cell pools or stable cell lines, following puromycin selection.
Background: Influenza A virus (IAV) is a virus that causes the flu in birds and mammals, including humans. Influenza A viruses are negative-sense, single-stranded, segmented RNA viruses whose subtypes are labeled according to an H number (for the type of hemagglutinin) and an N number (for the type of neuraminidase). There are 18 different known H antigens (H1 to H18) and 11 different known N antigens (N1 to N11), classified based on their reaction to antisera. Hemagglutinin binds to sialic acid on the surface of the target cells, and it is thus critical for infection. Neuraminidase is an enzyme that cleaves sialic acid and allows newly formed viruses to be released from the infected cells. The alpha/beta T cell receptor (TCR) HA1.7 specifically recognizes the influenza A virus hemagglutinin (HA) peptide (PKYVKQNTLKLAT) presented by the allo-major histocompatibility complex (MHC) class II molecule. Influenza is responsible for more than 36, 000 deaths/year in the USA only, and costs about 10 billion of dollars. It is thus crucial to continue to investigate and develop tools to minimize the impact of this virus on human health.
Description: The Influenza A Virus HA TCR Lentivirus (Clone HA1.7) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles transduce cells with human TCR (T-cell receptor) clone HA1.7 that specifically recognizes the Influenza A virus (or IAV) HA (hemagglutinin) peptide (PKYVKQNTLKLAT) in the context of MHC (major histocompatibility complex) class II molecules. The Influenza A Virus HA TCR α chain and β chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker.
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: ≥2 x 107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Biosafety Level: BSL-2
References: Hennecke J. and Wiley D.C., 2002 J Exp Med. 195:571-581.
Hennecke J., et al., 2000 EMBO J. 19:5611-5624.
Lamb J.R., et al., 1982 Nature. 300:66-69.
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