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Applications: Retroviral and lentiviruses-mediated transduction.
Background: Transduction is defined as the process by which foreign DNA is introduced into a cell by a virus or a viral vector. This method of DNA delivery was discovered in 1952 in Salmonella and transduction with viral vectors is now an essential tool in molecular biology. Four different types of viral vectors are commonly used: retrovirus, lentivirus, adenovirus and adeno-associated virus. The use of transduction enhancers is common practice as a method to increase efficiency, particularly in cell types known to be hard to transduce, such as primary cells, by improving the cell-virus contact. Both the surface of viral particles and the sialic acid on the cell plasma membrane are negatively charged, which decreases the chance of contact and entrance of the viruses into the cells. Cationic compounds like Polybrene (hexadimethrine bromide) can neutralize the negative charges and allow a closer proximity between the cells and the viruses. The use of transduction enhancers has become an essential tool in cell and gene therapy.
Description: Lenti-Fuse™ Polybrene Viral Transduction Enhancer is an easy to use preformulated polybrene solution designed to enhance the transduction efficiency of retroviruses or lentiviruses. Polybrene is a cationic polymer that acts by neutralizing the charge repulsion between the virus and the cell surface (both negatively charged), thus increasing the transduction efficiency.
Storage Stability: Store at -20°C for up to 2 years.
Supplied As: Each vial contains 500 µl at 10 mg/ml in water.
Warnings: Avoid freeze/thaw cycle.
Biosafety Level: Not applicable (BSL-1)
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