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Applications: Screen for activators or inhibitors of Myc signaling pathway in transduced target cellsGenerate Myc Luciferase Reporter stable cell line (puromycin resistant)
Background: The Myc signaling pathway plays an important role in cell proliferation, differentiation, transformation and apoptosis. c-Myc is a transcription factor that heterodimerizes with Max to regulate Myc signaling pathway-responsive genes. Genetic alterations in MYC have been linked to a number of human cancers, including Burkitt's lymphoma, cervical, ovarian, breast, lung and pancreatic carcinomas. Thus, Myc is a promising therapeutic target for cancer treatment. Myc can be activated by the Wnt/β-catenin pathway.
Description: The Myc Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the Myc response element located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the Myc signaling pathway in the target cells can be monitored by measuring the luciferase activity.
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer >107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
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