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Application: Lentiviral-mediated primary transduction.
Background: Transduction is defined as the process by which foreign DNA is introduced into a cell by a virus or a viral vector. This method of DNA delivery was discovered in 1952 in Salmonella and transduction with viral vectors is now an essential tool in molecular biology. Four different types of viral vectors are commonly used: , adenovirus and adeno-associated virus. The use of transduction enhancers is common practice as a method to increase efficiency, particularly in cell types known to be hard to transduce, such as primary cells, by improving the cell-virus contact. Transduction of primary NK cells is notoriously challenging, requiring extensive protocol optimization. Their resistance to transduction has been a major handicap in the development of NK-based immunotherapies, particularly at large scale. Optimization efforts have been focusing on the use of specific lentivectors, such as baboon envelope pseudotyped lentivectors, and feeder cells. An easy to use, scalable solution brings major benefits to the manufacture of CAR (chimeric antigen receptor)-NK cells.
Description: NK Viral Transduction Enhancer is a preformulated non-toxic transduction enhancer composed of components A and B, designed to enhance primary NK cell transduction efficiency with lentiviruses.
Storage Stability: Store at -20°C for up to 2 years.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Colamartino A., et al., 2019 Front Immunol. 10:2873.
Allan D., et al., 2021 Mol Ther Methods Clin Dev. 20:559-571.
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