Products from BPS Bioscience require a minimum order value above 400€Applications: Generate a stable cell line expressing human NKp46 with puromycin selection
Background: NKp46 is an important NK-activating receptor expressed on the surface of human NK cells that is involved in natural cytotoxicity. NKp46 participates in the activation of NK cells against pathogens, tumor cells, and virally infected cells; it also plays an important role in autoimmune conditions, including type I and II diabetes. NKp46 expression is often conserved on infiltrating NK cells in most solid tumors, and NKp46 is also a diagnostic biomarker and possible therapeutic target for gastrointestinal T-cell lymphoproliferative diseases.
Description: The NKp46 Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. The particles contain a human NKp46 (NM_004829.7) driven by an EF1A promoter and a puromycin selection marker (Figure 1).
Figure 1: Schematic of the lenti-vector used to generate the NKp46 Lentivirus.
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥10
7 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Uniprot: O76036
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
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