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Application: Expression of human PTK7 in cells of interest.Generate PTK7-expressing cell pools or stable cell lines following puromycin selection.
Background: PTK7 (Protein Tyrosine Kinase 7) is a pseudokinase of the receptor tyrosine kinase (RTK) family and shares a common ligand (Wnt5a) with fellow pseudokinases ROR1 (receptor tyrosine kinase-like orphan receptor 1) and ROR2. Despite the lack of a functional kinase domain PTK7 still contributes to downstream signaling of Wnt5a through its interaction with LRP6 (low-density lipoprotein receptor-related protein 6) and FZD (frizzled) receptors. PTK7 expression contributes to oncogenic potential in a variety of solid tumors including ovarian, colorectal and breast cancers and neuroblastoma. The cell surface expression and oncogenic function of PTK7 in a variety of tumor types makes it a compelling target for both CAR (chimeric antigen receptor)-T cell and antibody based therapeutic development. The use of cofetuzumab pelidotin, an ADC (antibody-drug conjugate) based on auristatin and targeting PTK7, showed promise in the treatment of ovarian cancer, NSCLC (non-small cell lung cancer) and TNBC (triple-negative breast cancer), showing the potential of targeting PTK7 in oncology.
Description: The PTK7 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with human PTK7 (Protein Tyrosine Kinase 7) (NM_002821.5) driven by a CMV promoter. The lentiviruses also transduce a puromycin selection marker.
Formulation: The lentivirus particles were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
References: Dessaux, C., et al., (2024) Oncogene 43, 1973-1984.
Jie Y., et al., (2021) Front. Immunol. 12:665970.
Lee J.Y., et al., (2023) Cell Rep Med 4(6):101091.
Maitland M.L., et al., (2021) Clin Cancer Res 27 (16): 4511-4520.
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