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Applications: Screen for activators or inhibitors of the STAT6 signalingGenerate STAT6 Luciferase Reporter cell pools or stable cell lines by puromycin selection.
Background: STAT6 (signal transducer and activator of transcription 6) is a member of the STAT family of proteins which activate gene expression by relaying signals from cytokines that bind to their receptors on the cell surface to the nucleus. STAT6 is mainly activated by IL-4 (interleukin-4) and IL-13, via phosphorylation by JAKs (Janus kinases). Once phosphorylated, STAT6 forms a homodimer that translocate to the nucleus and binds to DNA via the DNA-binding domain, activating gene transcription. STAT6 signaling is involved in T-helper type 2 (Th2) cell development and differentiation, macrophage activation, and it impacts the levels of CD20 on the surface of B lymphocytes. Gene fusions products of STAT6 with NGFI-A-binding protein 2 (NAB2) are responsible for fibrous tumors. Dysfunction of the STAT6 pathway leads to allergic reactions (asthma, atopic dermatitis, food allergies) and cancer (Hodgkin, central nervous system and follicular lymphoma). STAT6 can serve as a diagnostic and prognostic tool. Inhibitors have been shown to reduce acute inflammation in animal models, and future developments may lead to new therapeutical tools for STAT6 related pathologies.
Description: The STAT6 Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most mammalian cells, including primary and non-dividing cells. These viruses contain a firefly luciferase reporter driven by multiple copies of STAT6 (signal transducer and activator of transcription 6) responsive elements located upstream of the minimal TATA promoter. The lentiviruses also contain a puromycin selection marker. After transduction, the cellular STAT6 signaling pathway can be monitored by measuring the luciferase activity.
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer >107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
References: Kim M., et al., 2018 Cell Physiol Biochem 45(5):1863-1877.Hu X.,et al., 2021Signal Transduction and Targeted Therapy 6: 402.Karpathiou G.,et al., 2021Pathol Res Pract. 223:153477.
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