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Applications: Expression of Trop-2 in cells of interest.Generate stable cell lines expressing cynomolgus Trop2 (puromycin resistant).
Background: Trophoblast cell-surface antigen 2 (Trop-2, also known as tumor-associated calcium signal transducer 2, TACSTD2, GA733-1 or M1S1), is a cell surface glycoprotein involved in stem-cell like functions. It is overexpressed in a variety of solid cancers compared to normal cells, and links with patient prognosis. Through a variety of signaling pathways, Trop-2 regulates cancer growth and metastasis. The use of antibodies, antibody-fusion proteins, small molecule inhibitors and other strategies directed at Trop-2 has resulted in promising results. Its use as biomarker and target in cancer therapy shows its importance in the field.
Description: The Trop2 (Trophoblast cell-surface antigen 2) Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with Macaca fascicularis (also known as crab-eating macaque or cynomolgus monkey) Trop2 (XP_005543292.2) driven by an EF1A promoter. The lentiviruses also transduce a puromycin selection gene.
Formulation: The lentivirus particles were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer≥107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
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