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Applications: In combination with GAL4 DBD-GR lentivirus (BPS Bioscience #78632) to monitor glucocorticoid signaling pathway activity.Generate a UAS Luciferase Reporter stable cell line (puromycin resistant)
Background: GAL4/UAS is a binary gene expression system used for modeling expression vectors or transgenic animals with GAL4 and UAS constructs. UAS is a regulatory sequence critical to induction and the increased expression of a nearby gene of interest. GAL4 binds to UAS to activate transcription from a basal promoter located downstream of the activation sequence. Gal4/UAS is a highly advantageous system that can induce gene expression at a desired time and at higher levels than endogenous tissue-specific promoters. It can link a portion of a nuclear receptor to a reporter system to study ligand binding or to screen small molecules.GAL4 DBD-GR lentivirus (BPS Bioscience #78632), expresses a fusion protein where the glucocorticoid receptor (GR) ligand binding domain is fused to the DNA binding domain of GAL4 (GAL4 DBD). After co-transduction of UAS luciferase reporter lentivirus and GAL4 DBD-GR lentivirus. The glucocorticoid-induced activation of the glucocorticoid receptor can be monitored by measuring the luciferase activity.The glucocorticoid signaling pathway plays an important role in development, fluid homeostasis, cognition, immune response and metabolism. Glucocorticoids are a class of steroid hormones that bind to the glucocorticoid receptor, causing it to translocate to the nucleus. Upon translocation, the receptor can regulate the transcription of many genes, including those that regulate glucose metabolism and inflammatory responses.
Description: The UAS (Upstream Activation Sequence) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by a multimerized GAL4 upstream activation sequence (UAS) located upstream of the minimal TATA promoter and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the UAS-controlled signaling pathway in the target cells can be monitored by measuring the luciferase activity.
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer >107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
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