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Applications: Use to transduce cells with the goal of screening Vγ4Vδ1 TCR agonist antibodies.Use to transduce cells to be used as a positive control for Vγ4Vδ1 TCR evaluation and optimize experimental conditions.Generate Vγ4Vδ1 TCR expressing cell pools or stable cell lines, following puromycin selection.
Background: γδ TCRs (T cell receptors), αβ TCRs, and antibodies, result from gene rearrangements and offer the immune system the possibility to recognize several different types of antigens. γδ TCRs recognize antigens in a similar way to antibodies, being able to recognize full protein antigens and being independent on antigen binding to the MHC (major histocompatibility complex). γδ TCRs are cell type-specific, with Vγ4Vδ1 being present in γδTIL (tumor infiltrating lymphocytes) cells. Vδ1 expressing cells are found in mucosal and epithelial tissues and correspond to about 15% of the γδT cells present in PBMCs, with the % of γδ being only 5% of all the T cells. Vγ4Vδ1 cells can be activated by CD1 (cluster of differentiation 1) and respond to BTNL3 (butyrophilin-like 3), BTNL8, annexin A2 and A6. These cells can lead to tumor cell death by lysis. While most of the studies have been focusing on Vγ9Vδ2 T cells, a better understanding of the function and therapeutic potential of Vγ4Vδ1 T cells may open new avenues in cancer therapy.
Description: Vγ4Vδ1 TCR Lentivirus are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce a TCR (T Cell Receptor) which belongs to the Vγ4Vδ1 subtype of γδ TCRsd. The TCR γ chain and δ chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker.
Formulation: The lentiviruses were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the virus at -80°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: ≥2 x 107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
References: Allison T. and Garboczi D., 2002 Molecular Immunology 38 (14): 1051-1061.
Song Y., et al., 2022 Front Immunol 13: 914839.
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