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Applications: Screen for activators or inhibitors of the AhR signaling pathway.Generate a XRE luciferase reporter stable cell line (puromycin resistant) following puromycin selection and limiting dilution
Background: The aryl hydrocarbon receptor (AhR) functions as a sensor of xenobiotic chemicals, notably aromatic hydrocarbons, natural plant flavonoids, and plant polyphenolics, and it regulates stress pathways in eukaryotic cells. It is a cytosolic transcription factor that is normally kept inactive by binding to co-chaperones such as heat shock protein 90 (HSP90). Upon binding to xenobiotic chemicals, the chaperone dissociates from AhR, which results in AhR translocating into the nucleus and dimerizing with AhR nuclear translocator (ARNT). The heterodimer binds to a canonical XRE, regulating the transcription of target genes.
Description: The Xenobiotic response element (XRE) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by three copies of an XRE located upstream of the minimal TATA promoter (Figure 1), and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the activation of aryl hydrocarbon receptor (AhR) in the target cells can be monitored by measuring the luciferase activity.
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer >107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
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