Product Description: EasyBlot anti-Sheep IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of IgG and does not bind to the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP).
The protein A/G beads used in the IP procedure sometimes bind to IgG and produce contaminating signals at approximately 50 kDa and 25 kDa. This kit also includes an EasyBlocker (GTX425858) that can minimize the background caused by Protein A/G contamination. GeneTex’s EasyBlot kit is simple to use and detects only the target band in your IP experiments, resulting in cleaner western blots.
Application Note: Suggested starting dilutions for WB is 1:1000. Use 2-5 μg of the IP antibody; do not exceed 5 μg. Completely reduce all IP samples by 100mM DTT (fresh prepared). For further detail, please refer to the protocol.Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Positive Control: 0.2 μg native sheep IgG , 0.1 μg native sheep IgG
Concentration: 0.18 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species: Sheep
Target Isotype: IgG
Purification: Affinity purified by Protein G.
Conjugation: HRP
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