Application Note: Protein Molecular Weight Marker has been tested and is designed for use in accurate estimation of protein molecular weight in SDS-polyacrylamide gel electrophoresis and western blotting. Thaw the cocktail at room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 5-10µl is appropriate for most gel formats.
Concentration Value: 1X
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Liquid (in 1x Loading Buffer)
Purity and Specificity: Protein Molecular Weight Marker is supplied in 1X loading buffer and is ready-to-use. It contains: [62.5 mM Tris-HCl, pH 6.8, 2% (w/v) SDS, 250 mM NaCl, 1mM EDTA, 4% (v/v) β-Mercaptoethanol, 35% (v/v) Glycerol and 0.05% (w/v) bromophenol blue.] This product are unstained markers optimized for use with Coomassie blue staining, but the loading volume can be adjusted for compatibility with other staining methods (i.e. silver staining). The intensity of the 67 kDa bovine albumin band has been increased to serve as a reference.
Background: This protein molecular weight marker is a cocktail containing seven purified proteins that range in size from 14 to 120 kDa and thus span a molecular weight range common to most proteins and protein subunits. The protein mixture has been formulated to yield well-defined bands after SDS–polyacrylamide gel electrophoresis and staining with Coomassie blue or other common staining techniques.
Low Endotoxin: No
Other: User Optimized
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