Product Description: SignalPlus Antibody Enhancer for Western Blotting and ELISA enhances antigen-antibody reactions. SignalPlus Antibody Enhancer works well with chemiluminescence, colerimetric and fluorescence detection system. It can significantly enhance detection of weak immunoreactive and low abundance proteins in a variety of immunoassays such as Western blotting, dot blotting and ELISA. Simply dilute antibodies with SignalPlus Antibody Enhancer and process the rest of the procedures as usual. No additional steps are required. Signal enhancement is protein dependent and could vary from several folds to more than ten-fold.
Application Note: Dilute primary antibody in Solution 1 and secondary antibody in Solution 2. We recommend using a 10-fold lower working concentration of primary antibody than is usually required for western blot. Optimal working dilutions should be determined experimentally by the end user.