Product Description: The GTX49384 Trident Prestained Protein Ladder (Standard Range) is a ready-to-use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 180 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). GTX49384 Trident Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Application Note: Under suggested conditions, Trident Prestained Protein Ladder resolves 10 major bands in 15% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane. 5 μl per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel.
Form: Liquid
Buffer (with preservative): 20mM Tris-Phosphate pH7.5, 2% SDS, 0.2mM DTT, 3.6M urea, 15% (v/v) Glycerol, no preservative.
Concentration: 0.1~0.4 mg/ml (Please refer to the vial label for the specific concentration.)
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