Formulation: A solution in 1 mM Tris buffer (pH 7.5)
Formal Name: 2′-deoxy-5-[3-[[6-[[[3′,6′-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-5(or 6)-yl]carbonyl]amino]-1-oxohexyl]amino]-1-propen-1-yl]-uridine 5′-(tetrahydrogen triphosphate), trisodium salt
Purity: ≥95%
Formula Markup: C39H38N4O21P3 • 3Na
Formula Weight: 1060,6
Shelf life (days): 730
Notes: Fluorescein-12-dUTP is a fluorescently tagged form of deoxy-UTP that can be used to generate fluorescently labeled DNA via reverse transcription, nick translation, random primed labeling, or PCR. It displays ex/em maxima of 498/517 nm, respectively. Fluorescein-12-dUTP has been used to detect nucleic acids in situ.{57903} As a starting point for labeling, utilize approximately 0.05 µg/µl DNA, 25-50 U/ml DNA polymerase, and 10-100 µM fluorescein-12-dUTP in 10 mM Tris-HCl, pH 7.5, containing 1 mM EDTA, 5 mM NaCl, and 0.1 mM DTT, as well as dATP, dCTP, dGTP, and dTTP at 1 mM each in a total volume of 20 µl.
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