Formulation: Lyophilized from sterile 20 mM Tris, pH 7.5, 500 mM sodium chloride, and 10% glycerol
Purity: ≥95% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Autographa californica multiple nucleopolyhedrovirus (AcMNPV) major envelope glycoprotein is a class III viral fusion protein.{67202} AcMNPV is a double-stranded DNA insect virus and member of the Baculoviridae family. AcMNPV major envelope glycoprotein exists as a trimer and is composed of five domains in the low pH, post-fusion state that is highly post-translationally modified via glycosylation sites and a palmitoylation site at the C-terminus.{67203,67204} It is expressed on the surface of infected cells and budded virions.{67202} AcMNPV major envelope glycoprotein is involved in viral envelope-host cell endosome membrane fusion in a low pH-dependent manner and in virion budding.{67205,67206} Lipoplexes containing AcMNPV major envelope glycoprotein have been used for gene delivery to mammalian cells, which can be inhibited by an AcMNPV major envelope glycoprotein neutralizing antibody.{67207} Cayman’s AcMNPV Major Envelope Glycoprotein protein consists of 472 amino acids, has a calculated molecular weight of 54.2 kDa, and a predicted N-terminus of Ala21 after signal peptide cleavage.
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