Shelf life (days): 365.0
Formulation: 100 µg of biotinylated protein G-purified monoclonal antibody
Immunogen: Synthetic peptide from human vimentin with citrullines at 144 and 146 (G146R mutation)
Clone Designation: 12G11
Target specificity: Human
Cross Reactivity: + Citrullinated vimentin; - Unmodified vimentin
Uniprot ID: P08670
Formula Weight: 0.0
Notes: Vimentin is a cytoskeleton intermediate filament protein present in cells of mesenchymal origin, including leukocytes, endothelial cells, and smooth muscle cells. It is subject to citrullination by peptidyl arginine deiminase 2 (PAD2; Item No. 10785) under high calcium concentrations, which can occur during macrophage apoptosis.{31884} Citrullinated vimentin has been shown to have a role in the production of anti-citrullinated protein antibodies (ACPAs).{31883,31884} Serum levels of immune complexes targeting citrullinated vimentin are increased in patients with rheumatoid arthritis and positive for ACPAs.{66176} Serum and plasma levels of citrullinated vimentin are also increased in patients with lung cancer or idiopathic pulmonary fibrosis, respectively.{35094,35741} Cayman's Citrullinated Vimentin Monoclonal Antibody - Biotinylated (Clone 12G11) can be used for ELISA, immunoprecipitation (IP), and Western blot (WB) applications. The antibody recognizes citrullinated vimentin at approximately 54 kDa from human samples.
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