Formulation: 400 µl of CNBr-activated Sepharose 4B coupled to a human MR1 monoclonal antibody (Clone 2H7) (Item No. 34257)
Immunogen: Recombinant human MR1 (aa 22-292) protein
Clone Designation: 2H7
Shelf life (days): 365
Notes: Major histocompatibility complex (MHC) class I-related gene protein (MR1) is a non-polymorphic MHC class Ib antigen-presenting cell surface molecule that is required for T cell receptor-mediated activation of mucosal-associated invariant T (MAIT) cells.{60473,60472} It is composed of α1 and α2 domains, which form an antigen-binding pocket, and an α3 domain that interacts with β2-microglobulin.{60472} MR1 mRNA is ubiquitously expressed and, following translation, MR1 protein is localized to the endoplasmic reticulum in a partially folded state. Upon binding of a riboflavin-derived microbial antigen, MR1 undergoes a conformational change and translocates to the cell surface where it induces MAIT cell activation via an interaction with the MAIT cell T cell receptor and activates various immunomodulatory effects, including cytokine release, initiation of adaptive immune responses, and promotion of tissue repair.{60474} Cayman's MR1 Immunoaffinity Sorbent is designed for immunoprecipitation (IP) of MR1 protein from biological samples. This is an effective way to concentrate MR1 protein for subsequent detection by a different MR1 antibody by immunoprecipitation (IP) (LC/MS certified) and Western blot (WB). The MR1 affinity sorbent consists of Cayman's MR1 Monoclonal Antibody (Clone 2H7) (Item No. 34257) coupled to CNBr-activated Sepharose 4B.
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