Formulation: A solid
Formal Name: P′→5′-ester with 3-(aminothioxomethyl)-1-β-D-ribofuranosylpyridinium adenosine 5′-(trihydrogen diphosphate), inner salt
Purity: ≥98%
Formula Markup: C21H27N7O13P2S
Formula Weight: 679,5
Shelf life (days): 1460
CAS Number: 4090-29-3
Notes: Thio-NAD is a thione-modified derivative of the signaling molecule and enzyme cofactor NAD+ (Item No. 16077).{66388} It can replace NAD+ as a cofactor in alkaline phosphatase (ALP) enzyme activity assays. Thio-NAD has been used as a substrate in dual-enzyme cycling ELISA to identify severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein, also known as surface glycoprotein, and live M. tuberculosis bacterium in patient sputum.{66389,66390} It exhibits absorbance at 405 nm, allowing it to be distinguished from other cofactors.{66388}