Formulation: A solid
Formal Name: 3,3′,5,5′-tetramethyl-[1,1′-biphenyl]-4,4′-diamine
Purity: ≥98%
Formula Markup: C16H20N2
Formula Weight: 240,3
Shelf life (days): 1460
CAS Number: 54827-17-7
Notes: TMB is a colorimetric substrate for peroxidases, including hemoglobin, myeloperoxidase, and horseradish peroxidase (HRP).{3639,8109,8111,3371} Enzymatic oxidation of TMB produces a radical cation followed by a diimine product that can be quantified by colorimetric detection at 652 and 450 nm, respectively, as a measure of peroxidase activity. TMB has commonly been used as a substrate for HRP in ELISA and immunohistochemical applications.{47395,28302}