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Applications: Study enzyme kinetics, and screen small molecular inhibitors of histone demethylases for HTS applications in drug discovery.
Description: The 4x HDM Incomplete Assay Buffer does not contain -ketoglutarateand provides a more accurate background measure than using a no-enzyme control. Ithas been optimized for use with the following BPS Bioscience histone demethylaseassay kits:FBXL10 (KDM2B) Homogeneous Assay Kit, BPS Cat. #50610FBXL11 (KDM2A) Homogeneous Assay Kit, BPS Cat. #50611JARID1A (KDM5A) Homogeneous Assay Kit, BPS Cat. #50510JARID1B (KDM5B) Homogeneous Assay Kit, BPS Cat. #50512JARID1C (KDM5C) Homogeneous Assay Kit, BPS Cat. #50511JHDM1D (KDM7A) Homogeneous Assay Kit, BPS Cat. #50420JMJD1A (KDM3A) Homogeneous Assay Kit, BPS Cat. #50412JMJD2A (KDM3A) Homogeneous Assay Kit, BPS Cat. #50413JMJD2B (KDM4B) Homogeneous Assay Kit, BPS Cat. #50414JMJD2C (KDM4C) Homogeneous Assay Kit, BPS Cat. #50415JMJD2E (KDM4DL) Homogeneous Assay Kit, BPS Cat. #50417JMJD3 (KDM6B) Homogeneous Assay Kit, BPS Cat. #50416PHF8 (KDM7B) Homogeneous Assay Kit, BPS Cat. #50515UTX (KDM6A) Homogeneous Assay Kit, BPS Cat. #50516
Format: Aqueous buffer solution
Storage Stability: Stable for at least 1 year when stored as directed.
Supplied As: HEPES-based buffer containing reducing agent
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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