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Application: Study enzyme kinetics, and screen small molecular inhibitors of histone demethylases for HTS applications in drug discovery.
Description: The 4x HDM Incomplete Assay Buffer does not contain -ketoglutarateand provides a more accurate background measure than using a no-enzyme control. Ithas been optimized for use with the following BPS Bioscience histone demethylaseassay kits:/ FBXL10 (KDM2B) Homogeneous Assay Kit, BPS Cat. #50610/ FBXL11 (KDM2A) Homogeneous Assay Kit, BPS Cat. #50611/ JARID1A (KDM5A) Homogeneous Assay Kit, BPS Cat. #50510/ JARID1B (KDM5B) Homogeneous Assay Kit, BPS Cat. #50512/ JARID1C (KDM5C) Homogeneous Assay Kit, BPS Cat. #50511/ JHDM1D (KDM7A) Homogeneous Assay Kit, BPS Cat. #50420/ JMJD1A (KDM3A) Homogeneous Assay Kit, BPS Cat. #50412/ JMJD2A (KDM3A) Homogeneous Assay Kit, BPS Cat. #50413/ JMJD2B (KDM4B) Homogeneous Assay Kit, BPS Cat. #50414/ JMJD2C (KDM4C) Homogeneous Assay Kit, BPS Cat. #50415/ JMJD2E (KDM4DL) Homogeneous Assay Kit, BPS Cat. #50417/ JMJD3 (KDM6B) Homogeneous Assay Kit, BPS Cat. #50416/ PHF8 (KDM7B) Homogeneous Assay Kit, BPS Cat. #50515/ UTX (KDM6A) Homogeneous Assay Kit, BPS Cat. #50516
Format: Aqueous buffer solution
Storage Stability: Stable for at least 1 year when stored as directed.
Supplied As: HEPES-based buffer containing reducing agent
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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