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Application: Useful for biotinylation of proteins with free primary amine groups.
CAS Number: 365441-71-0
Contraindications: China
Description: Biotinylation reagent with N-hydroxysuccinimide, and very long (12 PEG) spacer. The NHS group reacts with the e-amino group on the N-terminus of proteins, and other primary amines. A coupling agent such as EDC is required to activate the reaction.
Format: White to off-white solid. May appear sticky.
Purity: ≥97%
Solubility: Soluble in DMSO
Storage Stability: Store at or below -20°C. Solid form is stable at least 2 years from date of receipt, when stored as directed.
Warnings: Protect from moisture. Store with desiccant.
Biosafety Level: Not applicable (BSL-1)
References: Application Reference(s):1. Takano, E., . et al. Pipette tip biosensors for bacterial double-stranded DNA using bioluminescence induced by zinc finger luciferase. Microchim Acta 184, 1595–1601 (2017). https://doi.org/10.1007/s00604-017-2152-0