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Applications: Useful as a substrate for CERK activity assays.
Description: Substrate for ceramide kinase (CERK), optimized for use with β-Octylglucoside mixed micellar assay.
Format: Aqueous buffer solution, lipid
Storage Stability: >6 months at -20°C when stored as components. Prepare substrate immediately before use.
Supplied As: A 2-component set containing sufficient reagents for 150 × 50 µl assays.Ceramide (0.75 mL @1.25 mM) Ceramide buffer (2 × 1.5 mL)
Warnings: Prepare substrate immediately before use. Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wijesinghe, D.S., et al., (2005). J. Lipid Res. 46(12):2706-16.
2. Van Overloop, H., et al., (2006). J. Lipid Res. 47:268-283.Application Reference:Biological characterization of 3-(2-amino-ethyl)-5-[3-(4-butoxyl-phenyl)-propylidene]-thiazolidine-2,4-dione (K145) as a selective sphingosine kinase-2 inhibitor and anticancer agent (2013)
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