Products from BPS Bioscience require a minimum order value above 400€
Applications: Study enzyme kinetics, and screen small molecule inhibitors of HDAC class2a for drug discovery and HTS applications.
Description: A fluorogenic, acetylated peptide substrate for class2a HDACs (HDACs 4, 5, 7 and 9). It also works well for HDACs 8 and 11. This substrate exhibits longer excitation and emission wavelengths (excitation 485 nm, emission 528 nm) than our regular class 2a HDAC substrate, #50040, which fluoresces at excitation 350-380 nm, emission at 440-460 nm. This substrate should be used when the inhibitor fluoresces near 450 nm. If autofluorescence is not an issue, we recommend product #50040. For autofluorescent samples using HDACs 1-3 ,6, and 10, we recommend substrate #50038.
Format: DMSO solution
Storage Stability: Stable at least 12 months from date of receipt, when stored as directed
Supplied As: 5 mM DMSO solution (50 µl)
Tags: Fluorescein
Warnings: Protect from direct exposure to light. Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. A. Ito et al. (2001) EMBO J. 20 1331.
2. A. Ito et al. (2002) EMBO J. 21 6236.
3. N.A. Barlev et al. (2001) Mol. Cell 8 1243.
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