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Applications: Study enzyme kinetics, and screen small molecule HDAC inhibitors for drug discovery and HTS applications.
Description: A fluorogenic, acetylated peptide substrate for HDACs. It is a suitable substrate for class I HDACs (HDACs 1, 2, and 3) and class 2b HDACs (HDACs 6 and 10). This substrate exhibits longer excitation and emission wavelengths (excitation 485 nm, emission 528 nm) than our regular class 1/2b HDAC substrate, #50037, which fluoresces at excitation 350-380 nm, emission at 440-460 nm. This substrate should be used when the inhibitor fluoresces near 450 nm. If autofluorescence is not an issue, we recommend HDAC substrate 3, #50037. For autofluorescent samples using HDACs 4, 5, 7, 8, 9, and 11, we recommend substrate #50042.
Format: DMSO solution
Storage Stability: Stable at least 12 months from date of receipt, when stored as directed
Supplied As: 5 mM DMSO solution (50 µl)
Tags: Fluorescein
Warnings: Protect from direct exposure to light. Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. A. Ito et al. (2001) EMBO J. 20 1331.
2. A. Ito et al. (2002) EMBO J. 21 6236.
3. N.A. Barlev et al. (2001) Mol. Cell 8 1243.Product Specific Reference(s): 1. Day, JA., et al., J Med Chem. 2013 Oct 24;56(20):7997-8007.
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