HDAC Assay Buffer

Products from BPS Bioscience require a minimum order value above 400€

Applications: Suitable for use with cell extracts, nuclear extracts, immunoprecipitates, and purified HDAC enzymes.

Description: HDAC assay buffer is an essential reagent for signal generation in HDAC assays performed with any of BPS Bioscience's HDAC fluorimetric substrates or assay kits. An appropriate fluorimetric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.) in HDAC assay buffer. Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC assay developer produces a fluorophore. Trichostatin A is included asan 'inhibitor stop' for class I and II HDACs.

Format: Aqueous buffer solution

Storage Stability: Stable at least 12 months from date of receipt, when stored as directed

Supplied As: Tris-buffered solution

Warnings: Do not dilute! Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

References: Application Reference:
Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014)

• SKU: BPS50031
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 50031
• Package Unit: 20 ml
• Quantity Unit: STK