Products from BPS Bioscience require a minimum order value above 400€Application: In vivo imaging of luciferase activity
Background: Luciferases are enzymes that produce light when they oxidize their substrate. Oxidation of l
uciferin, the substrate for the most commonly used firefly (Photinus pyralis) luciferase, results in bioluminescence that can be accurately measured using a luminometer and is proportional to the amount of luciferase enzyme present in the system under study. The firefly luciferase reaction requires luciferin, plus adenosine triphosphate (ATP) and Mg
2+ as cofactors, and when in the presence of O
2, emits a characteristic glow. Common biological experiments that utilize the luciferase reaction are promoter activity assays in which luciferase expression is under the control of a specific promoter, and cell line-derived xenograft (CDX) models in which constitutive luciferase expression allows for real-time, non-invasive monitoring of tumor growth and measurement of disease progression or drug efficacy.
Figure 1. Bioluminescent Reaction Catalyzed by Luciferase.
Description: The In vivo-Luc™ imaging solution is a ready-to-use, sterilized, endotoxin-free solution of D-Luciferin for in vivo imaging of the expression of firefly luciferase. Real-time disease progress or drug efficacy in animals can be monitored in a non-invasive manner using the In vivo-Luc™ imaging solution.
Formulation: 15 mg/ml D-Luciferin in Dulbecco's phosphate-buffered saline (DPBS) sterilized and endotoxin free.
Storage Stability: Reagents will perform optimally for up to 6 months from date of receipt when the materials are stored as directed. Upon first thaw, prepare single-use aliquots and store at -20°C. Luciferin is very sensitive to multiple freeze/thaw cycles. Discard any unused product after thawing from aliquots.
Warnings: Avoid freeze/thaw cycles. Protect from light.
Biosafety Level: Not applicable (BSL-1)
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