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Applications: Study of HDAC kinetics, regulation and inhibitor sensitivity. Suitable for use with the Kinetic HDAC assay kits:
Kinetic HDAC1 Assay Kit
Kinetic HDAC2 Assay Kit
Kinetic HDAC3 Assay Kit
Description: The Kinetic HDAC assay developer is an essential reagent for signal generation in Kinetic HDAC assays performed with any of BPS Bioscience's HDAC fluorimetric substrates. The developer sensitizes the deacetylated substrate so that subsequent treatment with the HDAC assay developer produces a fluorophore.
Format: Aqueous buffer solution
Storage Stability: Store in aliquots, only as the 10x concentrate, at -70°C. Concentrate is stable for at least 3 freeze-thaw cycles, but care should be taken that both freezing and thawing steps are done quickly (e.g. liquid N2 or dry ice/EtOH for freezing, rapid finger-rubbing for thawing).
Supplied As: A 10x concentrate (1 ml) with 10 ml dilution buffer.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. K. Ito et al. Proc. Natl. Acad. Sci. USA 2002 99: 8921
2. B. Heltweg and M. Jung Anal. Biochem. 2002 302: 175
3. S. Milutinovic et al. J. Biol. Chem. 2002 277: 20974
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