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Description: The Modified RIPA Lysis Buffer is intended for the lysis of mammalian adherent and suspension-cultured cells. This buffer enables the extraction of cytoplasmic and nuclear proteins in mild denaturing conditions. This is an SDS-free buffer optimized and formulated for proteins that might be sensitive to some components of the standard RIPA Buffer. This buffer is compatible with many applications, including ELISA-based assays.
Formulation: 50 mM Tris HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 0.5% Triton X-100, and 0.5% sodium deoxycholate
Mycoplasma Testing: Sterile filtered (0.2 µm)
Storage Stability: Upon receipt, store at 4°C. Stable for 6 months from date of receipt, when stored as directed.
Biosafety Level: Not applicable (BSL-1)