Type: Indirect.
Assay Time: 1.5h.
Detection Type: Colormetric.
Tested Sample Types: Serum, plasma.
Test Principle: This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the Monkeypox Virus (MPXV) IgG in the sample. The micro ELISA plate provided in this kit is precoated with purified MPXV antigen, after adding samples to wells, the MPXV IgG in the samples will combine with the pre-coated MPXV antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated goat anti human IgG to develop the antigenantibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain MPXV IgG and HRP conjugated anti-human IgG will appear blue in color. The enzymesubstrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether MPXV IgG exists in the tested samples or not.
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