Formulation: Lyophilized from sterile 20 mM Tris, pH 7.4, with 500 mM sodium chloride and 10% glycerol
Purity: ≥95% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Respiratory syncytial virus (RSV) fusion (F) protein is a surface glycoprotein encoded by the F gene in RSV RNA.{67138} It is synthesized as an inactive precursor protein, F0, that undergoes proteolytic cleavage to release the F1 and F2 subunits, which are joined together by two disulfide bonds.{67139} Mature RSV F protein is composed of an N-terminal fusion peptide (FP), two heptad repeats (HRs), a transmembrane domain, and a cytoplasmic tail and assembles into homotrimers on the virus surface.{67138} Upon insertion of the FP in the target cell membrane, the HRs form a six-helical bundle (6-HB) that enables RSV to fuse with the target cell. RSV F protein is highly conserved between RSV subtypes A and B with approximately 90% amino acid identities.{67140} RSV is the most common causative agent of pediatric lower respiratory tract infections.{67141} Cayman's RSV F Protein Extracellular Domain (Strain RSS-2) protein consists of 519 amino acids, has a calculated molecular weight of 57.8 kDa, and a predicted N-terminus of Phe22 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the F0 and F1 proteins is approximately 63 and 44-53 kDa, respectively, due to glycosylation.
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