Formulation: 50 mM Tris-HCl, pH 8.5, 10 mM calcium chloride
Purity: ≥90% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Secretory phospholipase A2 (sPLA2) (Type X) is a calcium-dependent PLA2 superfamily member that is encoded by PLA2G10 in humans.{56210} It is composed of an N-terminal signal peptide, a highly-conserved calcium-binding loop, a catalytic domain, sPLA2 Type I- and II-specific disulfides that provide stability, and a C-terminal extension. sPLA2 (Type X) is expressed in spleen, thymus, peripheral blood leukocytes, pancreas, lung, and colon, as well as neutrophils and keratinocytes.{4588,12334,10281} Upon activation, sPLA2 (Type X) is released into the extracellular space where it acts in a paracrine or autocrine manner and preferentially catalyzes the hydrolysis of phosphatidylethanolamine (PE) and phosphatidylcholine (PC) over phosphatidylserine (PS) at the sn-2 position, liberating the free fatty acid and lysophospholipid, which serve as substrates for the synthesis of bioactive lipid metabolites.{4588,56210} sPLA2 (Type X) increases arachidonic acid (Item Nos. 90010 / 90010.1 / 10006607) release from HEK293 cells and prostaglandin E2 (Item No. 14010) levels in HEK293 cells expressing human COX-2 but not COX-1.{7724} Pla2g10-/- mice have decreased inflammatory cell infiltration and airway edema in a model of ovalbumin-induced allergic asthma.{15028} Airway lining fluid levels of sPLA2 (Type X) are increased in patients with asthma.{68093} Cayman’s sPLA2 (mouse, recombinant Type X) protein can be used for enzyme activity assays. The specific activity of Cayman's sPLA2 (mouse, recombinant Type X) was established using Cayman’s sPLA2 Assay Kit (Item No. 765001) with 1.48 mM 1,2-bis(heptanoylthio) glycerophosphocholine (Item No. 62235) as the substrate.
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