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Encompassing Amino Acids: 658-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na orthovanadate, 0.1 mM ATP, 30 µg/ml Poly (Glu:Tyr)4:1 substrate, and 2 µg/ml recombinant Ret.
Description: Human Ret (GenBank Accession No. NM_020975), a.a. 658-end with N-terminal GST-tag, MW= 77.5 kDa, expressed in an Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 10 mM reduced glutathione, and 3 mM DTT.
Genbank: NM_020975
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P07949
Unit Definition: One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Moore,S.W., Appfelstaedt,J. and Zaahl,M.G. J. Pediatr. Surg. 42 (2), 326-332 (2007).
2 Griseri,P., Lantieri,F., Puppo,F., Bachetti,T., Di Duca,M., Ravazzolo,R. and Ceccherini,I. Hum. Mutat. 28 (2), 168-176 (2007).
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