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Encompassing Amino Acids: full length
Applications: Useful for protein binding studies and the study of biochemical function.
Background: Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
Description: Human Rheb (GenBank Accession No. NM_005614), full length with N-terminal His-tag, MW=23 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: NM_005614
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q15382
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Karbowniczek,M. et al. Biol. Chem. 281 (35), 25447-25456 (2006).
2. Buerger,C. et al. Biochem. Biophys. Res. Commun. 344 (3), 869-880 (2006).